FIG. 3.

(A to C) mRNA levels in AMs isolated from SM- and MC-infected C57BL/6J mice (five mice per group) as measured by RT-PCR showed that there were significant differences in PD-L1 (A), PD-L2 (B), and MHC-II (C) levels at 7 days (d-7) and 14 days postinfection. The data were obtained by comparison with the abundance of actin. (D) Flow cytometric analysis of AMs isolated from sham-, SM-, and MC-infected mice. The preparations were stained with PD-L1, PD-L2, and MHC-II Abs as described in Material and Methods. SM7 and MC7, SM- and MC-infected mice at 7 days postinfection, respectively; SM14 and MC14, SM- and MC-infected mice at 14 days postinfection, respectively. *, significant difference between SM and MC samples (P < 0.05) as determined by a t test (n = 5). The error bars indicate standard deviations.