TABLE 1.

RT-PCR primers and probes used in this study

Gene (species)	Primer (orientation) or probe	Sequence (5′-3′)
β-actin (human)	Forward	ACCGAGCGCGGCTACAG
	Reverse	CTTAATGTCACGCACGATTTCC
	Probe	(FAM)-TTCACCACCACGGCCGAGC-(TAMRA)
IL-1β (human)	Forward	CGAATCTCCGACCACCACTAC
	Reverse	TCCATGGCCACAACAACTGA
	Probe	(FAM)-AGGGCTTCAGGCAGGCCGC-(TAMRA)
IL-8 (human)	Forward	CTGGCCGTGGCTCTCTTG
	Reverse	CCTTGGCAAAACTGCACCTT
	Probe	(FAM)-CAGCCTTCCTGATTTCTGCAGCTCTGTGT-(TAMRA)
IFN-β (human)	Forward	TTCTCCACGACAGCTCTTTCC
	Reverse	CACTGACAATTGCTGCTTCTTTG
	Probe	(FAM)-TGAGCTACAACTTGCTTGGATTCC-(TAMRA)
GAPDH (chicken)	Forward	GCCGTCCTCTCTGGCAAAG
	Reverse	TGTAAACCATGTAGTTCAGATCGATGA
	Probe	(FAM)-AGTGGTGGCCATCAATGATCCC-(TAMRA)
IL-1β (chicken)	Forward	GCTCTACATGTCGTGTGTGATGAG
	Reverse	TGTCGATGTCCCGCATGA
	Probe	(FAM)-CCACACTGCAGCTGGAGGAAGCC-(TAMRA)
IL-8 (chicken)	Forward	GCCCTCCTCCTGGTTTCAG
	Reverse	CGCAGCTCATTCCCCATCT
	Probe	(FAM)-TGCTCTGTCGCAAGGTAGGACGCTG-(TAMRA)
IFN-β (chicken)	Forward	ACAACTTCCTACAGCACAACAACTA
	Reverse	GCCTGGAGGCGGACATG
	Probe	(FAM)-TCCCAGGTACAAGCACTG-(TAMRA)