FIG. 1.

PgmA is required for Y. pestis autoaggregation. (A to D) Overnight cultures grown at 28°C were transferred to a test tube and allowed to sit, without shaking, for autoaggregation at room temperature. The OD₆₂₀ values of the tubes were recorded at the indicated time points. The results are from duplicate assays of three independent experiments (n = 6). (E) Expression levels of PgmA-HA or PgmA-HA catalytic mutants (S160A and R521S) were determined by Western blotting using an anti-HA tag antibody directed against the C terminus of the hybrid protein. (F) Autoaggregation of identical numbers of Y. pestis KIM5 and the ΔpgmA mutant after a 45-min incubation at room temperature. Defects in autoaggregation were restored upon complementation with plasmid-encoded PgmA.