TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| AAEC185 | supE44 hsdR17 mcrA mcrB endA1 thi-1 ΔfimB-fimH ΔrecA | 4 |
| DH5α | supE44 ΔlacU169(F80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Laboratory collection |
| Y. pestis | ||
| KIM5-3001 | KIM5-3001 Pgm− Strr pCD1+ pCP1+ pMT1+; referred to as KIM5 in the text | 46 |
| KIM5-3001 ΔpsaA ΔyapC Δcaf1 | ΔpsaA ΔyapC Δcaf1 | 20 |
| KIM5-3001 ΔpgmA | ΔpgmA | This study |
| KIM5-3001 ΔyapC | ΔyapC | 21 |
| KIM5-3001Δail | Δail | 20 |
| KIM5-3001Δail ΔyapC | Δail ΔyapC | This study |
| KIM5-3001 ΔpgmA ΔyapC | ΔpgmA ΔyapC Kanr | This study |
| KIM5-3001 ΔpgmA Δail | ΔpgmA Δail | This study |
| KIM5 ΔpgmA ΔyapC Δail | ΔpgmA ΔyapC Δail Kanr Cmr | This study |
| KIM5-3001 pCD1− | pCD1− | 21 |
| KIM6+ | pPCP1+ pMT1+ pCD1− | 22 |
| KIM6+ ΔpgmA | ΔpgmA | This study |
| Plasmidsa | ||
| pFD1 | Kmr transposon and Himar1 transposase on an Ampr R6K-based suicide plasmid | 58 |
| pMMB207 | IPTG-inducible expression vector; Cmr | 50 |
| pMMB207-pgmAKIM5 | pMMB207 encoding the KIM5 pgmA gene; Cmr | This study |
| pMMB207-pgmE. coli | pMMB207 encoding the E. coli DH5α pgm gene; Cmr | This study |
| pMMB208 | IPTG-inducible expression vector; inverted polylinker relative to pMMB207; Cmr | 50 |
| pMMB208-pgmA-HA | pMMB208 encoding the HA-tagged KIM5 pgmA gene; Cmr | This study |
| pMMB208-pgmA-S160A-HA | pMMB208 encoding the HA-tagged KIM5 pgmA gene with mutation S160A; Cmr | This study |
| pMMB208-pgmA-R521S-HA | pMMB208 encoding the HA-tagged KIM5 pgmA gene with mutation R521S; Cmr | This study |
| pcDNA3-HA | HA-tagging vector; Ampr | 36 |
| pKD46 | Ampr; λ-RED recombinase expression plasmid | 17 |
| pKD4 | Kmr; template plasmid | 17 |
| pCP20 | Flp recombinase expression plasmid; Ampr, Cmr | 17 |
a
The origin of the pgm or pgmA gene is indicated by the subscript.