FIG. 5.

Bacterial flagellin is sufficient to trigger pore formation. DOTAP was used to transfect proteins into BMMs obtained from C57BL/6 and caspase-1^−/− (casp-1^−/−) mice. Cultures were stimulated for 6 h and assayed for pore formation. (A) Cultures were treated with flagellin alone, DOTAP alone, DOTAP and p131 from S. mansoni, or DOTAP and flagellin. (B) BMM cultures were treated with DOTAP and a mock-flagellin preparation (extracted from ΔflaA L. pneumophila), DOTAP and flagellin, or DOTAP and flagellin treated with proteinase K. Data are representative of three independent experiments. *, P < 0.05 in comparison to BMMs treated with DOTAP and flagellin.