FIG. 3.

Reporter analysis of TLR9 promoter variants in response to various stimulants. HEK293 cells were transiently transfected with promoterless pGl₃-Basic, TLR9-T-Luc, or TLR9-C-Luc. The transfection efficiency was normalized by cotransfection of a pRL-TK Renilla control plasmid. Cultures were stimulated with, TNF-α (0.5 ng/ml), serovar Typhimurium and H. pylori LPS (200 ng/ml) or CpG-DNA (5 μg/ml) for 16 h before the cells were lysed for luciferase measurements. The results are reported as the fold increase in RLA of the TLR9 constructs compared to the promoterless pGl₃-Basic vector. The data represent the mean ± the SEM of six experiments each performed in quadruplicate. The statistical significance of differences in luciferase activity between TLR9-T-Luc and TLR9-C-Luc was assessed by using the Student t test (unpaired) (*, P < 0.001, **, P < 0.025, ***, P < 0.01).