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. 2009 Dec 28;78(3):1345–1352. doi: 10.1128/IAI.01226-09

FIG. 4.

FIG. 4.

Noshift assays using HeLa nuclear extracts were performed to assess the difference in binding affinity to NF-κB between the two allelic variant promoters, 46C and 46T, using HeLa nuclear extracts. Competition studies with unlabeled 46C (N46C), 46T (N46T), and standard unlabeled NF-κB competitor probes (SP) were performed. Unlabeled probes were added 10 times in excess compared to 46C by using the Student t test (unpaired) (*, P < 0.01). The figure shows the means ± the SD of results obtained in three separate experiments.