FIG. 5.

46C NF-κB interaction analysis utilizing nuclear protein extracts obtained from HEK293 cells stimulated with TNF-α (0.5 ng/ml for 20 min), S. Typhimurium and H. pylori LPS (both at 200 ng/ml for 30 min), and CpG-DNA (0.5 μg/ml for 8.5 h). The percent increase in NF-κB of individual stimulation was calculated by normalizing to the negative, i.e., unstimulated, control. Binding activity is clearly observed between 46C and HEK293 NF-κB, and the amount of increase in NF-κB nuclear translocation correlates with the increase in luciferase activity, i.e., the TLR9 promoter activity, shown earlier. The figure shows the means ± the SD of results obtained in three separate experiments.