FIG. 2.

Comparison of the neutralization activities of the 5C12 antibody fragments to those of their parent IgG1 antibodies in a HeLa cell cytotoxicity assay. The percentage of increased survival of HeLa cells in the presence of 5 ng of Stx2 and various amounts of antibody or antibody fragments (0.8 to 25 ng) was determined. The percent survival values are from at least two independent assays (triplicate wells) and represent the mean of these values. Overall, the majority of the observed differences in neutralization activities were not statistically significant when analyzed by one-way ANOVA.