TABLE 2.
Neutralization activities of 5C12 IgG1 antibodies and 5C12 Fab fragments against Stx2 in the mouse toxicity model
| Antibody and dose (μg/mouse) | Survival rate (%)a | No. of days (mean ± SD)b | P valuec |
|---|---|---|---|
| PBS control | 0 | 1.96 ± 0.17 | |
| r5C12 IgG1 | |||
| 1.2 | 90 | 6.50 ± 1.59 | <0.0001 |
| 0.6 | 100 | 7.00 ± 0.00 | <0.0001 |
| 0.3 | 90 | 6.47 ± 1.67 | <0.0001 |
| 0.15 | 10 | 2.37 ± 1.63 | 0.5488 |
| r5C12 Fab | |||
| 5.0 | 0 | 1.99 ± 0.33 | 0.0210 |
| 2.5 | 0 | 2.45 ± 1.62 | 0.4082 |
| 1.2 | 0 | 2.01 ± 0.33 | 0.3676 |
| 0.6 | 0 | 1.88 ± 0.21 | 0.0789 |
| 5C12 HuMAb | |||
| 1.2 | 70 | 5.48 ± 2.45 | 0.0007 |
| 0.6 | 80 | 5.95 ± 2.22 | 0.0007 |
| 0.3 | 30 | 4.23 ± 2.23 | 0.0042 |
| 0.15 | 10 | 2.46 ± 1.63 | 0.3544 |
| 5C12 Fab | |||
| 5.0 | 0 | 2.63 ± 1.57 | 0.0210 |
| 2.5 | 0 | 2.11 ± 0.68 | 0.4082 |
| 1.2 | 0 | 2.45 ± 1.62 | 0.3676 |
| 0.6 | 0 | 2.03 ± 0.30 | 0.0789 |
a
Percentage of mice (10 per group) that survived through day 7. Mice euthanized on day 7 were given a survival score of 7 days.
b
Mean survival (days) for each group.
c
P values were calculated for the comparison of the mean survival of the PBS control group and each treated group by parametric (log rank test) analysis.