TABLE 3.
Neutralization activities of 5H8 IgG1 antibodies and Fab fragments against Stx2 in the mouse toxicity model
| Antibody and dose (μg/mouse) | Survival rate (no. of surviving mice/total no. of mice [%])a | No. of days (mean ± SD)b | P value for control group vs treated groupc |
|---|---|---|---|
| Control | 0/10 (0) | 2.25 ± 0.42 | |
| r5H8 IgG1 | |||
| 10.0 | 9/10 (90) | 8.05 ± 2.14 | <0.0001 |
| 5.0 | 10/10 (100) | 8.73 ± 0.00 | <0.0001 |
| 2.5 | 6/10 (60) | 6.40 ± 3.01 | <0.0001 |
| 1.2 | 0/10 (0) | 2.87 ± 0.83 | 0.0370 |
| 0.6 | 1/10 (10) | 3.23 ± 2.02 | 0.0673 |
| r5H8 Fab | |||
| 10.0 | 0/10 (0) | 1.91 ± 0.15 | 0.0247 |
| 5.0 | 0/10 (0) | 2.10 ± 0.28 | 0.3493 |
| 5H8 HuMAb | |||
| 10.0 | 10/10 (100) | 8.73 ± 0.0 | <0.0001 |
| 5.0 | 7/10 (70) | 6.96 ± 2.95 | 0.0001 |
| 2.5 | 7/10 (70) | 7.03 ± 2.75 | <0.0001 |
| 1.2 | 4/10 (40) | 4.86 ± 3.38 | 0.0571 |
| 0.6 | 1/10 (10) | 3.43 ± 2.12 | 0.0525 |
| 5H8 Fab | |||
| 10.0 | 0/10 (0) | 2.24 ± 0.39 | 0.8088 |
| 5.0 | 0/10 (0) | 2.01 ± 0.29 | 0.1469 |
a
Percentage of mice (10 per group) that survived through day 8. Mice euthanized on day 8 were given a survival score of 8 days.
b
Mean survival (days) for each group.
c
P values were calculated for the comparison of the mean survival of the control group and each treated group by parametric (log rank test) analysis.