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. 2010 Jan 11;78(3):976–983. doi: 10.1128/IAI.01012-09

FIG. 4.

FIG. 4.

Effects of complement deficiencies on opsonophagocytosis and whole-blood killing of S. pneumoniae. (A) Proportion of HL60 cells associated with fluorescent S. pneumoniae after incubation in sera from wild-type and complement-deficient mice. The results collected from three different experiments are expressed as means plus standard deviations (error bars). Values that are significantly different are indicated as follows: *, P < 0.001 compared with values determined for the complement deficiencies in Bf/C2 and Bf groups; #, P < 0.05 compared with values for the C1qa−/− group; **, P < 0.05 compared with values for the Bf/C2−/− group. (B) A representative histogram of fluorescence indicates, in order from left to right, the peak values for the blank, Bf/C2−/− mice, Bf−/− mice, C1qa−/− mice, and wild-type mice. (C and D) Killing of S. pneumoniae type 6A (C) and type 14 (D) with whole blood collected from wild-type and complement-deficient C1qa, Bf, and Bf/C2 mice. The data collected from three different experiments are expressed as means ± standard deviations (error bars) of the percent survival calculated as mean number of CFU after 60 and 120 min divided by the number of CFU in the inoculum at start × 100. Values that are significantly different are indicated as follows: *, P < 0.001 for the comparison of wild-type versus Bf/C2−/−, Bf−/−, and C1qa−/− cohorts; +, P < 0.01 for the comparison of C1qa−/− versus Bf/C2−/− or Bf−/− cohort; #, P < 0.05 for the comparison of Bf−/− versus Bf/C2−/− cohort.