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. 2010 Jan 19;78(3):963–975. doi: 10.1128/IAI.00925-09

FIG. 2.

FIG. 2.

Complementation of a region I kps deletion restores virulence. Competitive infections were performed with WT strain UTI89 attλ::PSSH10-1 (spectinomycin resistant) and the isogenic region I capsule deletion mutant UTI89 Δkps attHK::COM-GFP (kanamycin resistant; GFP expressing) for 48 h in C3H/HEN (TLR4+) mice. (A) Each strain carried the control vector pBAC-LacZ (designated VC). (B) UTI89 attλ::PSSH10-1 (WT) carries control vector pBAC-LacZ, and UTI89 Δkps attHK::COM-GFP carries complementing vector pBAC-LAC RegI kps, containing the entire region I capsule gene cluster under the control of its native promoter (designated C). A total of 10 mice were infected. Horizontal bars represent the geometric means. Statistical comparisons were performed using the Mann-Whitney nonparametric test.