TABLE 2.
Primers used in this study
| Primer | Sequence (5′-3′) | Reference |
|---|---|---|
| kpsF::EP185 #1 | GACCAGAGCTCTACTATCGA | This study |
| kpsF::EP185 #2 | GCAGGTACCATTCGCCATAT | This study |
| kps KO #1 | GTTACAACCCATTGATTTAGCATAAATAAATTATAGTGGGTTCGGGTTTGTTGTGTAGGCTGGAGCTGCTTC | This study |
| kps KO #2 | TGGAAATGATTTTTTGGCTACTTAAAATTCAAAAGATATTGACTTGAAATATGGGAATTAGCCATGGTCC | This study |
| kps flank #1 | ATGTTCCCGGTGGTCAACATGCTTCCAGCACTCCTT | This study |
| kps flank #2 | CCTCTTTGCACGATAAAAGGATTTTCTTG | This study |
| neu KO #1 | CCTATAGTGGTTACATTCCAATATTATGCCTTGGAAATATTTAACTGAGACATATCGTGTAGGCTGGAGCTGCTTC | This study |
| neu KO #2 | CTACTTTAAGATTTAATTTTACGACTGGTACTGTAATAGAATATAAAATGTAGCTTTTAATGGGAATTAGCCATGGTCC | This study |
| neu flank #1 | GTGCGGAACTGTCAAACCCGCTCAC | This study |
| neu flank #2 | AGACGCAGGTTAATTGGGTTTATTATGGG | This study |
| PRegI #1 | GACAATCGATTATCGGCGCA | This study |
| PRegI #2 | TTGGATCCTATCAGCTCCTT | This study |
| cdiA KO #1 | GCTCTTTTGTCATATTGATGTTTCCAATCAATTTACGTAAGGTAAGTGTAGGCTGGAGCTGCTTC | This study |
| cdiA KO #2 | ACGGTTTACTGGCGCGTTGCCGTCGCGTTTTAAGGGATTATTACCATGGGAATTAGCCATGGTCC | This study |
| cdiA flank #1 | GGTTGACCGGCCAGTCTGCTATGA | This study |
| cdiA flank #2 | CCAGAAGAACGGCTGCGTATTGC | This study |
| nanR flank #1 | CTTGCTTCTTTAACTGAAGCTGAACGTGAA | This study |
| nanR flank #2 | TCTGCTTCACTAAAGTGGCATTATTTCT | This study |