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. 2009 Dec 14;78(3):1109–1122. doi: 10.1128/IAI.00363-09

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FIG. 1. — qRT-PCR determination of relative transcriptional changes for hmbR (A), hemO (B), and tbpB and lbpB (C) in the misR/S mutants. Total RNAs were isolated from mid-log-phase cultures that were treated (gray bars) or not treated (black bars) with 100 μM dipyridyl for 45 min. The relative transcriptional differences between the mutants and the wild-type strain were calculated by the method (30), using the transcriptional level of the wild-type strain under iron-rich conditions as the calibrator. Each qRT-PCR was performed in triplicate and was repeated with at least two independent RNA preparations. The asterisks indicate statistically significant differences between the wild-type strain and the mutant as determined by the Student t test with a two-tailed distribution (P < 0.01). WT, wild type; comp., complemented.

Inline graphic — qRT-PCR determination of relative transcriptional changes for hmbR (A), hemO (B), and tbpB and lbpB (C) in the misR/S mutants. Total RNAs were isolated from mid-log-phase cultures that were treated (gray bars) or not treated (black bars) with 100 μM dipyridyl for 45 min. The relative transcriptional differences between the mutants and the wild-type strain were calculated by the method (30), using the transcriptional level of the wild-type strain under iron-rich conditions as the calibrator. Each qRT-PCR was performed in triplicate and was repeated with at least two independent RNA preparations. The asterisks indicate statistically significant differences between the wild-type strain and the mutant as determined by the Student t test with a two-tailed distribution (P < 0.01). WT, wild type; comp., complemented.