FIG. 2.

In vivo expression of the vv21574-vv21582 (brp) locus. The indicated strains were grown in HIN. RNA was isolated from mid-exponential (ME)- and stationary (S)-phase cells of the parental ATCC 27562 strain carrying pBAD24T (lane 1) or pBAD24T::dcpA (lane 2) and from an R variant, ATCC 27562R, with pBAD24T (lane 3). RT-PCR was used to determine if vv21574, vv21577, vv21578, and vv21580 were being transcribed. Expressions of the dcpA and the ribosomal rplT genes were used as positive controls. Primer sets were optimized for PCR using genomic DNA from V. vulnificus strain ATCC 27562, and PCR was performed on RNA samples to verify that they did not contain residual contaminating DNA. Target genes are indicated above the gels. M, 100-bp marker.