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. 2010 Jan 11;78(3):1390–1402. doi: 10.1128/IAI.01188-09

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FIG. 4. — c-di-GMP-induced biofilm and aggregate formation is dependent on the vv21574-vv21582 (brp) locus. The indicated strains were grown in HIN. (A) Biofilm (top panel) and aggregate (middle panel) formation in culture tubes by the parental, mutant (Δvv21574 and Δvv21580), and complemented (Δvv21574/pSU38T::vv21574 and Δvv21580/pSU38T::vv21580) strains carrying the pBAD24T control vector or pBAD24T::dcpA. Control strains for complementation studies carried the empty pSU38T plasmid. Colony morphologies for the same strains are shown in the bottom panel. (B) Quantitative measurement of biofilm formation of the corresponding strains in panel A by CV staining. Results shown are representative of at least three independent experiments. Error bars represent standard deviations.