FIG. 5.

Expression of the brp locus is required for biofilm and rugose colony formation by the R variant. The indicated strains were grown in HIN. (A) Biofilm formation in culture tubes (top panel) and colony morphology (bottom panel) of the parental and ATCC 27562R strains and the ATCC 27562RΔbrpF and ATCC 27562RΔbrpI mutant strains carrying the pSU38T vector control. Complemented strains carry pSU38T::brpF or pSU38T::brpI, respectively. (B) Quantitative measurement of biofilm formation of the corresponding strains in panel A by CV staining. Results shown are representative of at least three independent experiments. Error bars represent standard deviations.