Sensitivity of the parental, ATCC 27562R, ATCC 27562RΔbrpF, and ATCC 27562RΔbrpI strains to chlorine and increasing salinity. The indicated strains were grown in LB containing 100/00 NaCl. (A) The strains were then incubated in PBS containing 3 ppm NaOCl for 5 min. The surviving bacteria were enumerated by plate counts. The number of viable bacteria relative to that obtained following treatment with PBS alone is plotted. (B) Strains were grown in LB containing 00/00 (white bars), 20/00 (dark gray bars), 100/00 (light gray bars), and 300/00 (black bars) NaCl. Bacterial growth (OD595) is plotted. Results shown are representative of at least three independent experiments. Error bars represent standard deviations.