TABLE 2.
Primers used in this study
| Primer | Sequencea |
|---|---|
| PCR | |
| wcrFfwd | CCGCAATTGACCATGAGAATTTTACATATTATCAATG |
| wcrFrev | CCGTCTAGAAGCTCAATACAGGCAACCCT |
| wcrIfwd | CCGGAATTCATGAAAAAAGTACTGCATATTACA |
| wcrIrev | CCGTCTAGATCAAATATAGAGGCTGCTGAG |
| ΔwcrFfwd | CCGGGATCCGCTGGCCAGACATTGTTCAT |
| ΔwcrFrev | CCGTCTAGAAGCTCAATACAGGCAACCCT |
| ΔwcrIfwd | CCGGAATTCCAAAACTTATCTATACCCCACA |
| ΔwcrIrev | CCGTCTAGACTTTGTCACGCAACTGCTGTT |
| ptacKpnI | CCGGGTACCGTATAAGTGTGGAATTGTGAGC |
| ptacEcoRI | CCGGAATTCGTATAATGTGTGGAATTGTGAGC |
| ptac-wcrFfwd | TTCACCAACAAGGACCATAGCATATGAGAATTTTACATATTATCAATG |
| ptac-wcrFrev | CATTGATAATATGTAAAATTCTCATATGCTATGGTCCTTGTTGGTGAA |
| ptac-wcrIfwd | TTCACCAACAAGGACCATAGCATATGAAAAAAGTACTGCATATTACA |
| ptac-wcrIrev | TGTAATATGCAGTACTTTTTTCATATGCTATGGTCCTTGTTGGTGAA |
| RT-PCR | |
| vvuL20RT1 | GCTCGTGCACGTCATAAGAA |
| vvuL20RT2 | GACGGTCACGGTAAGCGTAT |
| dcpART1 | CAAGCTTTATGCCCTCGATG |
| dcpART2 | CATCCACAAGCCATTGACAGT |
| wcrART1 | CGTGGCGTACCAGTTCAATTCGA |
| wcrART2 | GTTTAATCCCAAGCGACGGAGGT |
| wcrDRT1 | TGGCTGGACAGTAATGCCAGACA |
| wcrDRT2 | GGTTTTGTGCGGATTTTCGCAGT |
| wcrFRT1 | GAAGGTGCAGGAAGAGTTGA |
| wcrFRT2 | TAGATAACCAGCGTTGCCGA |
| wcrIRT1 | AGAAGCATTTGGCGGTGGTGTAC |
| wcrIRT1 | TCACATCCGGTTGGAGTTGCTTG |
a
Restriction sites are in bold.