FIG. 5.

TDM induces NETs in the absence of fibrinogen. Sections of 7-day-old TDM matrices from Fib KO mice were labeled using rabbit anti-human neutrophil elastase and mouse anti-human histone H1, followed by Alexa Fluor 555 goat anti-rabbit and Alexa Fluor 488 goat anti-mouse antibodies and the DNA stain Draq5, and then examined by confocal immunofluorescence microscopy. (A) Histone H1 is observed along the surface of the streaming DNA. (C) Neutrophil elastase is observed within intact neutrophils (arrows) as well as along the streaming neutrophil DNA. (E) Draq5 staining indicates that this streaming material is composed of DNA. (B and D) Negative control sections were stained only with secondary antibody for H1 and elastase, respectively.