FIG. 1.

Potential translocation of L. pneumophila Ankyrin proteins by attached extracellular bacteria. Untreated or cytochalasin D-treated U937 cells were infected with the WT strain of L. pneumophila expressing the indicated Cya hybrid proteins. After 30 min of infection, cultured cells were lysed and cAMP was quantified by enzyme-linked immunosorbent assay (ELISA) and the amount of cAMP is indicated as fmol/well. The experiment was performed three times. The data points are the average of cAMP concentration for one representative experiment performed in triplicate. Error bars represent standard deviations of triplicate samples.