Skip to main content
. 2009 Dec 22;78(3):1123–1134. doi: 10.1128/IAI.00913-09

FIG. 3.

FIG. 3.

The ANK repeats and the C terminus of L. pneumophila AnkJ are required for delivery into host cell. (A) Organization of the AnkJ and different ANK repeats or C terminus in-frame deletions. Each protein size and different truncation is displayed. (B) Immunoblots of whole-cell bacterial extracts expressing the indicated Cya hybrid were probed with monoclonal antibody specific to the M45 epitope and reprobed with anti-CAT as a loading control. The numbers represent the different Cya-AnkJ truncated hybrid proteins: Cya-AnkJ (lane 1), Cya-AnkJΔA1 (lane 2), Cya-AnkJΔA2 (lane 3), Cya-AnkJΔA3 (lane 4), Cya-AnkJΔA1ΔA2 (lane 5), Cya-AnkJΔA1ΔA3 (lane 6), Cya-AnkJΔA2 ΔA3 (lane 7), Cya-AnkJΔA1ΔA2 ΔA3 (lane 8), and Cya-AnkJΔ259-269 (lane 9). (C) U937 cells were infected with the WT strain or the dotA mutant of L. pneumophila expressing the indicated Cya hybrid proteins. After 1 h of infection, cultured cells were lysed, and the cAMP was quantified by ELISA. The amount of cAMP is indicated as fmol/well. The experiment was performed three times, and the data are the average of the cAMP concentration for one representative experiment performed in triplicate. Error bars represent standard deviations of triplicate samples.