The L. pneumophila ankH and ankJ mutants are defective in the A/J mice model. (A) Groups of 30 mice were infected with 107, 108, 8 × 108, or 109 CFU of the L. pneumophila WT strain or the ankH or ankJ mutant compared to the ankB mutant. At 1, 2, 3, 4, and 5 days postinfection, mortality was determined. After 3 days there was no mortality. (B) A/J mice were infected with 106 CFU of L. pneumophila WT strain or the ankH or ankJ mutant and compared to the ankB mutant. At 1, 2, 3, and 7 days postinfection, three mice were sacrificed, and the lungs were collected for CFU enumeration.