FIG. 7.

Colocalization of AnkH and AnkJ with endosomal, lysosomal, Golgi, and ER compartments. Stable transfected HEK293 cells expressing GFP, the GFP-AnkH or GFP-AnkJ fusion proteins were fixed and stained with anti-Lamp2, anti-cathepsinD, anti-KDEL, anti-GM130, and anti-p58k antibodies. (A) Their association with endosomal, lysosomal, and ER compartments was assessed by confocal laser scanning microscopy. (B) Quantitation of colocalization. The results shown are representative of three independent experiments performed in triplicate. The data represent means ± the standard deviations.