TABLE 1.
ANK deletion constructs and primers used to generate ANK deletions in ankH and ankJ
| Construct | Primera |
|
|---|---|---|
| Orientation | Sequence (5′-3′) | |
| ankHΔA1 | F | CCAGACGTCACAGGACGC |
| R | TTCATCGATATCATCCAAAG | |
| ankHΔA2 | F | TACACTCGTAATGGTCTTTG |
| R | GACGTCTGGCTTGTTGATAT | |
| ankHΔ1391-1401 | F | TTAATTAGGATTAATCCCACAATCATCCAGAATT |
| R | TAACCCGTAAAGGAAATAATTTATT | |
| ankJΔA1 | F | TGATGCTATTTTCATTTC |
| R | TACGCCCCCCCCACTTATG | |
| ankJΔA2 | F | GGGGGGGGCGTACTGATT |
| R | CCTATCAACTATCACAAAG | |
| ankJΔA3 | F | ATAGTTGATAGGATAATC |
| R | CTGGTGAATAGCACCAATAT | |
| ankJΔ797-807 | F | TTATTCTTCAAAACGACTCTCTGGAAC |
| R | GGAGAAATACCTCCTTCAAGAA | |
a
All primers are 5′-phosphorylated. Orientation: F, forward; R, reverse.