TABLE 2.
Cya-Ank reporter constructs and primers used to generate ank fusions
| Construct | Primera |
Restriction site | |
|---|---|---|---|
| Orientation | Sequence (5′-3′) | ||
| pCya-ankB | F | GGATCCTTATGAAAAAGAATTTTTTTTCTG | BamHI |
| R | CTGCAGTTAACAAACAAGGCACTTGCT | PstI | |
| pCya-ankC | F | CCCGGATCCTTATGGATTTTGTAAGTGAAATGAA | BamHI |
| R | CCCCTGCAGTTACTATTTTAGGACAACTCGT | PstI | |
| pCya-ankD | F | CCCGGATCCTTATGTTGACTCCTCCGCCTGACT | BamHI |
| R | CCCCTGCAGTTAGTCCTGAGGATTTTCTTTA | PstI | |
| pCya-ankG | F | CCCGGATCCTTCTGAATTCATTATGGATAGC | BamHI |
| R | CCCCTGCAGTTATTTCATACCAAAACGAG | PstI | |
| pCya-ankH | F | CCCGGATCCTTATGAGTATTGCAAAC | BamHI |
| R | CCCCTGCAGTTATAGGCCTGTCGCAACAGGAT | PstI | |
| pCya-ankI | F | CCCGGATCCTTATGATTATTTTATATGATTTT | BamHI |
| R | CCCCTGCAGTTAAAAAAACTTGCTTTCAAGTGTGAT | PstI | |
| pCya-ankJ | F | CCCGGATCCTTGTGATTAAAATGGGTAGA | BamHI |
| R | CCCCTGCAGTTAAAGTGCGTTTTTAGGGGTATCTA | PstI | |
| pCya-ankK | F | CCCGGATCCTTATGCCTAGAGTTTATAATCTTA | BamHI |
| R | CCCCTGCAGTTAGATTTTATTCTTTGATAGTGATA | PstI | |
| pCya-ankN | F | CCCGGATCCTTGGTAAAAATTATGCC | BamHI |
| R | CCCCTGCAGTTACTACCATTTTAATTTCAAG | PstI | |
| pCya-ankQ | F | CCCGGATCCTTATGCTTATGGCCG | BamHI |
| R | CCCCTGCAGTTATGCTTATGGCCGCAACAA | PstI | |
a
Restriction sites are indicated in boldface. Orientation: F, forward; R, reverse.