TABLE 3.
GFP/3×Flag-Ank fusion constructs and primers used to generate ank fusions
| Construct | Primera |
Restriction site | |
|---|---|---|---|
| Orientation | Sequence (5′-3′) | ||
| p3×Flag-ankH | F | AGATCTGATGAGTATTGCAAACGATA | BglII |
| R | GGATCCTTATAGGCCTGTCGCAACAGGATT | BamH | |
| p3×Flag-ankJ | F | AAGCTTGTGATTAAAATGGGTAGA | HindIII |
| R | AGATCTTTAAAGTGCGTTTTTAGGGGTATC | BglII | |
| pAcGFP-ankH | F | AGATCTGATGAGTATTGCAAACGATA | BglII |
| R | TCTAGATTATAGGCCTGTCGCAACAGGATT | XbaI | |
| pAcGFP-ankJ | F | AGATCTGTGATTAAAATGGGTAGA | BglII |
| R | TCTAGATTAAAGTGCGTTTTTAGGGGTATC | XbaI | |
a
Restriction sites are indicated in boldface. Orientation: F, forward; R, reverse.