FIG. 5.

P. aeruginosa directly binds in vitro to isolated HS and N-glycan chains in a dose-dependent manner. Ninety-six-well plastic plates were coated overnight with increasing concentrations of the indicated molecules. GFP-conjugated bacteria were added for 1 h, and fluorescence was quantified in a fluorescence plate reader. Control bacterial binding to noncoated wells was set as a background, and the percentage of binding above that of the control is indicated. Shown are means ± SD for four separate experiments. HS, heparan sulfate; CS-4, 4-0-sulfated chondroitin sulfate; CS-6, 6-0-sulfated chondroitin sulfate; HA, hyaluronic acid; GlcN, N-acetylglucosamine.