FIG. 5.

Inhibition of excision and rescue by compound A. (A) Increasing concentrations of compound A up to 100 μM were added during a rescue reaction. The compound inhibited rescue by the wild-type NS5B enzyme with an IC₅₀ of 1.7 ± 0.3 μM, while the enzymes with the G152E and P156L mutations required doses of compound A that were at least an order of magnitude higher. (B) Compound A was also added during an excision reaction to determine its effects directly on pyrophosphorolysis. Data points are the means ± standard deviations for three independent experiments.