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. 2009 Dec 22;54(3):1029–1041. doi: 10.1128/AAC.00963-09

FIG. 4.

FIG. 4.

Effects of KCl on resistance to antibiotics and on release of proteins into culture medium. (A) Growth curve analyses of A. baumannii cells challenged with antibiotics in MHB (solid lines) or MHB supplemented with 150 mM KCl (dashed lines) at the indicated concentrations. Error bars represent the mean ± 1 SD and may be obscured by the symbol in some cases. Asterisks indicate statistically significant changes in growth upon antibiotic challenge in medium containing KCl compared to medium lacking KCl as determined by a Student's t test (*, P < 0.05; **, P < 0.005; ***, P < 0.0005). (B) SDS-PAGE of TCA-precipitated proteins from A. baumannii culture supernatants. A. baumannii cells were grown to stationary phase in MHB supplemented with 50 mM, 86 mM, 154 mM, or 308 mM KCl. *, bands that increased with increasing concentrations of KCl.