TABLE 1.
Distribution of mutations in the rpoB gene among 242 MDR isolates from tuberculosis cases in Shanghai, China
| Locus | Nucleotide change | Amino acid change | No. (%) of isolates (n = 242) |
|---|---|---|---|
| rpoB531 | TCG→TTG | Ser→Leu | 143 (59.1) |
| TCG→TGG | Ser→Trp | 2 (0.8) | |
| TCG→TTT | Ser→Phe | 1 (0.4) | |
| rpoB531 and rpoB526 | TCG→TGG and CAC→AAC | Ser→Trp and His→Asn | 1 (0.4) |
| TCG→GGG and CAC→CGC | Ser→Gly and His→Arg | 1 (0.4) | |
| rpoB526 | CAC→GAC | His→Asp | 13 (5.4) |
| CAC→TAC | His→Tyr | 15 (6.2) | |
| CAC→CGC | His→Arg | 6 (2.5) | |
| CAC→AAC | His→Asn | 3 (1.2) | |
| CAC→CCC | His→Pro | 1 (0.4) | |
| CAC→CTC | His→Leu | 1 (0.4) | |
| rpoB526 and rpoB511 | CAC→AAC and CTG→CCG | His→Asn and Leu→Pro | 2 (0.8) |
| rpoB526 and rpoB533 | CAC→AAC and CTG→CCG | His→Asn and Leu→Pro | 2 (0.8) |
| rpoB526 and rpoB534 | CAC→AAC and GGG→CGG | His→Asn and Gly→Arg | 1 (0.4) |
| rpoB526 and rpoB529 | CAC→CGC and CGA→CTG | His→Arg and Arg→Leu | 1 (0.4) |
| rpoB516 | GAC→GTC | Asp→Val | 12 (5.0) |
| GAC→CCC | Asp→Pro | 1 (0.4) | |
| rpoB516 and rpoB511 | GAC→GGC and CTG→CCG | Asp→Gly and Leu→Pro | 1 (0.4) |
| GAC→GTC and CTG→CCG | Asp→Gly and Leu→Pro | 2 (0.8) | |
| rpoB516 and rpoB518 | GAC→GGC and CTG→CCG | Asp→Gly and Leu→Pro | 1 (0.4) |
| rpoB516 and rpoB533 | GAC→GCC and CTG→CCG | Asp→Ala and Leu→Pro | 1 (0.4) |
| rpoB533 | CTG→CCG | Leu→Pro | 7 (2.9) |
| rpoB533 and rpoB515 | CTG→CCG and ATG→GTG | Leu→Pro and Met→Val | 1 (0.4) |
| rpoB533 and rpoB518 | CTG→CCG and ATG→GTG | Leu→Pro and Met→Val | 1 (0.4) |
| rpoB513 | CAA→CCA | Gln→Pro | 4 (1.7) |
| CAA→AAA | Gln→Lys | 1 (0.4) | |
| rpoB513 and rpoB511 | CAA→CCA and CTG→CCG | Gln→Pro and Leu→Pro | 2 (0.8) |
| rpoB515 and rpoB511 | ATG→CTG and CTG→CCG | Met→Leu and Leu→Pro | 1 (0.4) |
| rpoB522 | TCG→TGG | Ser→Trp | 1 (0.4) |
| TCG→TTG | Ser→Leu | 3 (1.2) | |
| rpoB519 | AAC→AAG | Asn→Lys | 1 (0.4) |
| Wild type | 9 (3.7) |