TABLE 1.

Oligonucleotide primers used in this study

Name	Use	Sequence (5′ to 3′)
AcrB[+]	SNP sequencing, cloning	GCC TGA ACA GTC CAA GTC TAG ACT TAA ACA GGA GCCa
AcrB[−]	SNP sequencing, cloning	GGT TTT CGT ATG GGA TCC TGA GTT GGT GGb
AcrA[+]	SNP sequencing	GCA CGA CGA TAA TCT AGA CGC AGC AAT GG
AcrA[−]	SNP sequencing	CCG GCA GTT TGA GGA TCC CCA GCC CCC CTG CC
AcrBqRT[+]	Expression	CTG ATC ATC GTG GTC GGC ATG GC
AcrBqRT[−]	Expression	CCA GTC CTT CAA GGA AAC GAA CGC
AcrAqRT[+]	Expression	CCA ATA TCG CGC AAT TGA CGG TGA ATC G
AcrAqRT[−]	Expression	GCG CAG GAA GTC GTT GCT GGA CTG G
GyrBqRT[+]	Expression normalization	CAG ACT GCC AGG AAC GCG AT
GyrBqRT[−]	Expression normalization	AGC CAA GCG CGG TGA TAA GC

^aUnderscored and boldface letters represent the XbaI site used for directional cloning into the pSportI plasmid vector.

^bUnderscored and boldface letters represent the BamHI site used for directional cloning into the pSportI plasmid vector.