FIG. 4.

Intracellular CoA pools of strain JT7 treated with N5-Pan (B) or left untreated (A). Major peaks determined by internal standards included in each run are as follows: a, a mixture of β-alanine, pantothenate, and malonyl-CoA; b, CoA; c, succinyl-CoA; and d, acetyl-CoA. Peak a includes all labeled compounds that failed to bind to the HPLC column (probably mainly β-alanine and pantothenate that was not removed by the preparatory C₁₈ column) and was highly variable from run to run. Note that malonyl-CoA, which was also included in peak a, is only a trace component of the CoA pools (11, 12). Peak e is an artifact of an unknown structure formed upon addition of N5-Pan to minimal M9 medium containing β-[3-³H]alanine. Its formation occurred in both the presence and the absence of cells.