FIG. 2.

Stage-dependent inhibition of HIV-1 replication by individual drugs. Infection of PM1 cells with NL4-3 and drug treatments were carried out as described in the legend to Fig. 1. For each of four stages of viral replication, two or more drugs were individually added during ongoing replication at 5 days after infection. p24 measurements were performed with samples collected 6 days after infection (1 day after drug addition). Each data point represents, for any given drug, the average of the means of three independent experiments, each of which was performed in duplicate or triplicate wells, relative to the results for the no-drug controls. Horizontal lines represent the averages of the means of all drugs targeting that stage of replication. Unpaired two-tailed t tests were used to test for statistically significant differences between the no-drug controls and each treatment group. For no drug versus entry inhibitors, P = 0.0061; for no drug versus RT inhibitors, P = 0.0059; for no drug versus IN inhibitors, P = 0.0021; for no drug versus PR inhibitors, P < 0.0001.