FIG. 5.

Kinetics of reverse transcription, integration, and stage-dependent inhibition of HIV-1 replication during single-round infections. PM1 cells were infected by spinoculation with NL4-3-deltaE-EGFP pseudotyped with the AD8 envelope (capable of only a single round of infection) and washed extensively to remove unbound virus. Early and late reverse transcription products (A) and integrated DNA and 2-LTR circles (B) were quantified by qPCR. (C) The RT inhibitor EFV or NVP or the IN inhibitor RAL or MK-2048 was added individually at defined time points after infection of PM1 cells by the same virus. The percentage of GFP-positive cells was determined at 48 h p.i. by flow cytometry and is expressed relative to that for the no-drug control. Data represent the means ± standard errors of the means of three independent experiments and were fitted to sigmoidal dose-response (variable slope) curves.