TABLE 3.

Diagnostic accuracy at the codon level: results of initial and repeat hybridizations compared to sequencing (validation at external sites)

Codon	No. (%) of tests with indicated resulta
	Full concordanceb				Partial concordance			IND on LiPA	Complete discordance	Total
	Total	WT	MUT	MIX	MIX LiPA no MIX Seq	MIX Seq no MIX LiPA	Different MIX
L80V/I	39	34	3	2	11	0	0	0	0	50
V/G173L	44	41	3	0	5	0	0	1	0	50
L180M	32	14	18	0	18	0	0	0	0	50
A181T/V	49	48	1	0	1	0	0	0	0	50
M204V/I/S	32	10	21	1	14	0	3	1	0	50
N236T	48	48	0	0	2	0	0	0	0	50
Total	244 (81.3)	195 (65.0)	46 (15.3)	3 (1.0)	51 (17)	0 (0)	3 (1.0)	2 (0.7)	0 (0)	300 (100)

^aWT, wild type; MUT, mutant; MIX, wild type and mutant both present with respect to codons 80, 173, 180, 181, 204, and 236; Mix LiPA no MIX Seq, detection of both wild type and mutant by LiPA versus either wild type or mutant detected by sequencing; MIX Seq no MIX LiPA, detection of both wild type and mutant by sequencing versus either wild type or mutant detected by LiPA; Different MIX, different mixture between LiPA and sequencing of both wild type and mutant present with at least one shared amino acid by both methods; IND on LiPA, indeterminate result with LiPA.

^bThe data for full concordance between LiPA and direct sequencing are divided into three groups: the WT group, MUT group, and MIX group.