TABLE 5.
Details of overall results after initial and repeat hybridizations for partially concordant results
| Codon | No. of results with indicated mutation(s)a |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| WT Seq |
MUT Seq |
MIX Seqb |
Total | ||||||
| WT + MUT LiPA |
WT + MUT LiPA |
MUT LiPA | WT + MUT LiPA | MUT LiPA | WT LiPA | ||||
| Total | Total | ||||||||
| L80V/I | 17 (V), 5 (V+I), 2 (I) | 24 | 4 (V), 6 (V+I), 1 (I) | 11 | 7 (V+I) | 0 | 0 | 0 | 42 |
| V/G173L | 14 | 14 | 3 | 3 | 0 | 0 | 0 | 0 | 17 |
| L180M | 9 | 9 | 37 | 37 | 0 | 0 | 11 | 0 | 47 |
| A181T/V | 6 (T), 4 (V) | 10 | 2 (T), 1 (V) | 3 | 0 | 1 (T+V)3 | 0 | 12 | 15 |
| M204V/I/S | 5 (V), 3 (I), 2 (V+I) | 10 | 10 (V), 2 (V+I), 3 (I) | 15 | 19 (V+I) | 1 (V+I)5 | 1 (V)4,5, (V+I)6,7-10 | 0 | 51 |
| N236T | 4 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 5 |
| Total | 71 | 70 | 26 | 2 | 7 | 1 | 177 | ||
a
WT Seq, wild-type codon detected by sequencing; MUT Seq, mutant codon detected by sequencing; MIX Seq, wild-type and mutant codons both present with respect to codons 80, 173, 180, 181, 204, 236; WT + MUT LiPA, detection of both wild type and mutant by LiPA versus either wild type or mutant detected by sequencing; MUT LiPA, two or more mutant codons detected by LiPA; WT LiPA, wild type detected by LiPA.
b
Superscripted numbers refer to results that showed a mix of wild types and mutants as detailed in Table 6.