TABLE 1.
Findings from database-based anti-HIV-1IIIB peptide screening
| Namea (source) | Peptide sequence (no. of residues)i | EC50(μM)j | TC50(μM)j | TI |
|---|---|---|---|---|
| AZT | 0.009 | >0.5 | >55.6 | |
| D70 (frog) | GWFDVVKHIAKRF-NH2 (13) | 2.99 | 10.6 | 3.55 |
| Metalnikowin I (insect) | VDKPDYRPRPRPPNM (15) | >54.4 | >54.4 | |
| Ranatuerin 9 (frog) | FLFPLITSFLSKVL (14) | 16.7 | 34.6 | 2.07 |
| Mastoparan M (insect)b | INLKAIAALAKKLL (14) | >67.6 | >67.6 | |
| D74 (insect)c | IKWKKLLRAAKRIL-NH2 (14) | >57.6 | 1.3 | <0.02 |
| Hyposin-5 (frog) | FRPALIVRTKGTRL (14) | >61.4 | >61.4 | |
| D76 (frog)d | FFGKVLKLIRKIF-NH2 (13) | 0.63 | 6.78 | 10.8 |
| Apidaecin IA (insect) | GNNRPVYIPQPRPPHPRI (18) | >47.4 | >47.4 | |
| Drosocin (insect)e | GKPRPYSPRPTSHPRPIRV (19) | >45.5 | >45.5 | |
| PGLa (frog) | GMASKAGAIAGKIAKVALKAL-NH2 (21) | >50.8 | >50.8 | |
| Clavanin B (tunicate) | VFQFLGRIIHHVGNFVHGFSHVF (23) | 7.1 | 37.1 | >5.18 |
| Buforin II (toad) | TRSSRAGLQFPVGRVHRLLRK (21) | >41.1 | >41.1 | |
| Styelin A (tunicate) | GFGKAFHSVSNFAKKHKTA-NH2 (19) | >48.5 | >48.5 | |
| Ponericin L2 (insect) | LLKELWTKIKGAGKAVLGKIKGLL (24) | 1.4 | 24.7 | 17.6 |
| Spinigerin (insect) | HVDKKVADKVLLLKQLRIMRLLTRL (25) | 3.05 | >33.3 | >10.9 |
| Piscidin 3 (fish) | FFHHIFRGIVHVGKTIHRLVTG (22) | 2.1 | 6.9 | 3.3 |
| Pseudin 1 (frog) | GLNTLKKVFQGLHEAIKLINNHVQ (24) | 35.7 | >36.8 | >1.03 |
| Misgurin (fish) | RQRVEELSKFSKKGAAARRRK (21) | >40.0 | >40.0 | |
| D88 (frog)f | NLVSGLIEARKYLEQLHRKLKNRKV (25) | >33.3 | >33.3 | |
| Lycotoxin I (spider) | IWLTALKFLGKHAAKHLAKQQLSKL (25) | >35.2 | 2.4 | <0.07 |
| Parasin I (fish) | KGRGKQGGKVRAKAKTRSS (19) | >50.0 | >50.0 | |
| Brevinin-2 related (frog) | GIWDTIKSMGKVFAGKILQNL-NH2 (21) | 1.65 | 7.42 | 4.49 |
| Maculatin 1.3 (frog) | GLLGLLGSVVSHVVPAIVGHF-NH2 (21) | 4.0 | 8.02 | 2.01 |
| Latarcin 3a (spider) | SWKSMAKKLKEYMEKLKQRA (20) | 32.7 | >40.3 | >1.23 |
| D94 (bacteria)g | GAWKNFWSSLRKGFYDGEAGRAIRR (25) | >34.1 | >34.1 | |
| Ascaphin-8 (frog) | GFKDLLKGAAKALVKTVLF-NH2 (19) | 1.2 | 2.9 | 2.4 |
| Desertcolin 1 (frog) | GLADFLNKAVGKVVDFVKS-NH2 (19) | >49.9 | >49.9 | |
| Melectin (insect) | GFLSILKKVLPKVMAHMK-NH2 (18) | 4.34 | 7.75 | 1.79 |
| D98 (frog)h | SLSRFLRFLKIVYRRAF-NH2 (17) | 0.83 | 8.6 | 10.4 |
| Isracidin (cow) | RPKHPIKHQGLPQEVLNENLLRF (23) | >36.2 | >36.2 |
The peptides were chosen from the APD (20). D70 is a mutant in which the last three residues of uperin 7.1 were changed from SAV to KRF. AZT, zidovudine.
C-terminal amidation is absent.
A peptide mutant of polybia-MPI with the following mutations: D2K, D8R, and Q12R.
A peptide mutant of temporin-PTa with S4K, P10R, and L13F mutations.
Residue T11 is not O glycosylated.
The sequence of this peptide corresponds to chain A of distinctin with residue C23 changed to R.
The sequence of this peptide corresponds to chain A of plantaricin JK.
A mutant of temporin-LTc with three mutations: S7R, P14R, and P15R.
The number of amino acid residues in the peptide is indicated in parentheses. C-terminal amidation is represented by NH2. Mutated residues are listed in bold.
The standard error of results from multiple antiviral assays is on average less than 10% of the respective mean EC50 or TC50.