FIG. 2.

Inhibitory activities of compounds 1 and 2 against the NS5B enzyme isolate panel. Gray bars represent compound 1, and white bars represent compound 2. Inhibitory activities determined using a primer-dependent RdRp assay are reported as n-fold changes in the IC₅₀ relative to the IC₅₀ for NS5BΔC21 1b J4. The 1a_2, 1a_6, 1b_9, and 1b_10 enzyme isolates were previously described, and the other enzymes were cloned and overexpressed as described previously (38). Compounds with a change in the IC₅₀ of >10-fold were considered to be less active (dotted line). The experiment was performed between 1 and 10 times in quadruplicate. Fold changes are reported as median values ± interquartile ranges.