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Coimmunoprecipitation of UL16 and UL21 from infected cells. Vero cells infected with HSV (A) or HSV.UL16-CFP (B) were disrupted in NP-40 lysis buffer 20 h postinfection. Proteins were immunoprecipitated (IP) with antibodies specific for UL11, UL16, UL21, or GFP or no herpesvirus proteins (preimmune [pre]). The proteins were separated by SDS-PAGE and analyzed by immunoblotting with anti-UL16 or anti-UL21 serum. A portion (1/15) of the lysate was analyzed for protein expression.
