FIG. 5.

Nuclear localization of GFP-UL21 in the absence of other viral proteins. Confocal microscopy results for GFP-tagged UL21 constructs are shown. Vero cells were seeded on coverslips and subsequently transfected with the indicated GFP-UL21 constructs. After 20 h, the cells were stained with Hoechst dye to label the nuclei, washed, paraformaldehyde fixed, affixed to slides, and examined with a confocal microscope to visualize the GFP signals alone (left panels) or in combination with the nuclear signals (right panels). For the bottom panels (below the dotted line), cells that had been transfected for 18 h were infected with HSV and examined at the indicated times postinfection (PI).