FIG. 6.

The second half of UL21 is sufficient for packaging into virions. Vero cells were transfected to express the indicated GFP-UL21 constructs or GFP alone and then infected with HSV at 24 h posttransfection. After another 20 h, extracellular virions were purified from the medium by centrifugation through a sucrose cushion. Virions and infected cell lysates were separated by SDS-PAGE and analyzed by immunoblotting with antibodies specific for GFP and VP5.