FIG. 8.

NEM modification of UL16 blocks the interaction with UL11 but not UL21. Insect cells infected with a baculovirus that expresses UL16-GFP were lysed with NP-40 buffer and either untreated (lane 1 of each panel) or treated for 30 min with NEM, either before or after 2 h of incubation with the indicated bead-bound GST-fusion protein (lanes 2 and 4 of each panel). Alternatively, NEM was added to the GST-fusion protein and washed away prior to mixing with UL16-GFP (lane 3 in each panel). The bead-bound GST fusion and associated proteins were washed, dissolved in sample buffer, separated in SDS-10% polyacrylamide gels, and visualized by immunoblotting with anti-GST (bottom) and anti-GFP (top) antibodies.