FIG. 4.

Biochemical footprinting confirmation of PTB binding site. Polyacrylamide gel analysis was performed on primer extension products obtained from transcripts digested with the single-strand-specific RNase T2 in the presence of PCBP2 (A) or GST, GST-PTB, or buffer alone (B). A sequencing ladder was run alongside, obtained using the same primer, to highlight the sequence protected by PCBP2 and PTB binding (boxed). (C) Schematic representation of SL3 highlighting the PCBP2 and PTB binding site identified by footprinting analysis.