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. 2009 Dec 30;84(6):3121–3126. doi: 10.1128/JVI.02002-09

FIG. 5.

FIG. 5.

The P204L and R237L mutations in the AD8 strain of HIV-1 confer resistance to AME. (A) 293T cells were transfected with WT pAD8 or the P204L and R237L AD8 mutants constructed by QuikChange site-directed mutagenesis (Stratagene). One day posttransfection, viral lysates were prepared as described in the legends to Fig. 1 and 3 and were subjected to immunoblotting with the 2F5 anti-gp41 monoclonal antibody. In this experiment, NL4-3 and its AME-resistant derivatives (P203L and R236L) were included as controls. (B) TZM-bl cells were infected with virus stocks derived from WT NL4-3 or AD8 or the indicated mutants. Infections were carried out in the absence or presence of 1, 5, or 10 μM AME as described in the legend to Fig. 1. Data shown are means ± SEs of the results from three independent experiments performed in duplicate.