FIG. 3.

Enforced expression of dnEBNA1 does not affect the growth of EBV-negative Akata cells. (A) Ak(−)-dnEBNA1 cells were cultured in the absence (−) or presence (+) of Dox. Total cell lysates were prepared and subjected to Western blot analysis with EBV-immune human serum (reactive to dnEBNA1 and wt EBNA1) and anti-β-actin antibody. EBV-positive Akata cells served as a positive control for wt EBNA1 detection [EBV(+)Ak]. (B) Ak(−)-dnEBNA1 cells were cultured in the absence (−) or presence (+) of Dox. Viable cells were counted every 3 days, and the cells were split. The total numbers of viable cells derived from the initial cultures were calculated and plotted.