FIG. 8.

Knockdown of BHRF1 expression in P3HR-1 cells results in increased cell death. (A) P3HR-1 cells were transfected with oriP plasmid expressing BHRF1-shRNA (or control shRNA) and EGFP. Three days after transfection, EGFP-positive cells were sorted and subjected to Western blot analysis with anti-BHRF1 and anti-β-actin antibodies. (B) P3HR-1 cells transfected with BHRF1-shRNA (or control shRNA) and EGFP were stained with annexin V-PE 4 days after transfection. The histograms represent annexin V staining after gating on EGFP-positive cells (top). The percentages of annexin V-positive cells among EGFP-positive cells are shown (bottom). Each bar represents the average of triplicate transfections, with the error bar representing the standard error of the mean.