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. 2010 Jan 13;84(6):3004–3015. doi: 10.1128/JVI.02459-09

FIG. 4.

FIG. 4.

Growth of EBOV/VP35KRA is strongly attenuated in 293T cells. Vero E6 and 293T cells were infected at an MOI of 0.01 with EBOVwt, EBOVwt/GFP, EBOV/VP35KRA, or EBOV/VP35KRA/GFP. (A) The replication of recombinant viruses expressing GFP was monitored using fluorescence microscopy on the indicated days postinfection (p.i.). Single GFP-expressing 293T cells observed after infection with EBOV/VP35KRA/GFP are indicated by arrows. (B) The culture medium from cells infected with either EBOVwt or EBOV/VP35KRA was harvested at different intervals postinfection and analyzed by Western blotting using polyclonal horse anti-EBOV antibodies. (C) Relative virus infectivity of virus stocks was estimated by normalization of the infectious titers (TCID50/ml) determined by virus titration on Vero E6 cells to the amount of viral proteins as quantified by Western blot analysis using rabbit anti-VP24 antibody and goat anti-rabbit antibodies. EBOV/VP35KRA is less infectious than wild-type EBOV.