FIG. 5.

HTLV-2 preinfection did not significantly affect either the viral load or the rate of CD4⁺ T-cell loss after SIV_mac251 infection. (A) Viral load in the lymph nodes, jejunum, and rectum in HTLV-2/SIV_mac251 coinfected macaques (black) and SIV_mac251 monoinfected macaques (white) quantified as the number of SIV DNA copies/10⁶ cells 10 and 30 days after SIV_mac251 infection. (B) The frequency of CD3⁺ CD4⁺ T cells in the bone marrow and lymph nodes of HTLV-2/SIV_mac251 coinfected and SIV_mac251 singly infected animals before infection (baseline) and 10 and 30 days after SIV_mac251 infection. (C) The frequency of CD3⁺ CD4⁺ T cells in the jejunum and rectum in HTLV-2/SIV_mac251 coinfected and SIV_mac251 singly infected animals before infection (baseline) and 10 and 30 days after SIV_mac251 infection. (D) Number of CD4⁺ expressing cells, enumerated by immunohistochemical staining of paraformaldehyde-fixed tissue sections from the rectum. A repeated-measure analysis of variance demonstrated that the difference between the baseline (before infection) values for HTLV-2/SIV_mac251 coinfected macaques and SIV_mac251 singly infected macaques is significantly different (P < 0.05). (E) Number of CD8⁺ expressing cells, enumerated by immunohistochemical staining of paraformaldehyde-fixed rectum tissue sections.